Rotubules typically overlap and are often densely packed inside cells. It

Rotubules typically overlap and are often densely packed inside cells. It is very difficult, if not impossible, to manually trace each individual microtubule in a confocal or widefield fluorescence microscopy image in order to obtain accurate estimates of microtubule distribution parameters. Hence previous work comparing cell lines has often focused on the tips of microtubules where tracing is possible, or the comparison has been only qualitative [7]. We therefore previously developed an indirect method for estimating natural, interpretable and quantitative parameters such as the number 25033180 and the mean length of microtubules from 3D fluorescence microscopy images of microtubules [8,9]. These parameters are important because they represent basic biophysical characteristics of tubulin polymerization. The basis of the method is to use a generative model of microtubule patterns (Figure 1) to synthesize 3D images for many values of the model parameters,Comparison of Microtubule Distributionsand then to pick the image that best matches the given real image (and thus to purchase 86168-78-7 estimate the parameters that could have produced it). Our original method utilized 3D images, but 3D images of intact whole cells are much less commonly available than 2D images. We therefore describe here a method of estimating 3D microtubule model parameters from 2D image fluorescence microscopy images of tubulin. We test our approach on the 3D images of HeLa cells previously used to develop the model, and then use it to compare microtubule distributions in different cell lines. Figure 2 provides an overview of the framework introduced in this paper. There are two sub-systems. One is for generating synthetic images of microtubules, and the other is for estimating the microtubule model parameters for real images through comparison with the synthetic images. We first obtained 2D fluorescence microscopy images for eleven cell lines. Each image contains two channels, one for microtubule MedChemExpress 374913-63-0 staining and the other for nuclear staining. The images are segmented to find individual cell and nuclear boundaries. For each cell, we estimate a Point Spread Function (PSF), centrosome location and single microtu-bule intensity. On the basis of the segmented 2D cell and nuclear shapes, approximate 3D cell and nuclear morphologies are generated. Given the model 23727046 (Figure 1) and ranges of allowed values of its parameters (number of microtubules (N), mean of the length distribution (mu) and collinearity (a)), synthetic images of microtubule distributions are generated for each 3D morphology for each combination of allowed parameter values. Each raw synthetic image is then convolved with the estimated PSF and multiplied with the estimated single microtubule intensity to make it comparable to the real image. Numerical features are then calculated on every real cell image and the synthetic images for it. The matching method then selects one set of parameters for which the synthetic image is the closest to the real image in the feature space. Using this indirect method, we estimate the model parameters for 2D images from eleven human cell lines, and analyze the resulting parameters.Results 3D Cell and Nuclear Shape Generation from a 2D Slice of Microtubule Channel and Nucleus ChannelIn our earlier work, we described an indirect approach to estimate parameters of a generative model of microtubules that was conditioned on the shape of the cell and the nucleus [8]. These shapes were estimated from a 3D confoca.Rotubules typically overlap and are often densely packed inside cells. It is very difficult, if not impossible, to manually trace each individual microtubule in a confocal or widefield fluorescence microscopy image in order to obtain accurate estimates of microtubule distribution parameters. Hence previous work comparing cell lines has often focused on the tips of microtubules where tracing is possible, or the comparison has been only qualitative [7]. We therefore previously developed an indirect method for estimating natural, interpretable and quantitative parameters such as the number 25033180 and the mean length of microtubules from 3D fluorescence microscopy images of microtubules [8,9]. These parameters are important because they represent basic biophysical characteristics of tubulin polymerization. The basis of the method is to use a generative model of microtubule patterns (Figure 1) to synthesize 3D images for many values of the model parameters,Comparison of Microtubule Distributionsand then to pick the image that best matches the given real image (and thus to estimate the parameters that could have produced it). Our original method utilized 3D images, but 3D images of intact whole cells are much less commonly available than 2D images. We therefore describe here a method of estimating 3D microtubule model parameters from 2D image fluorescence microscopy images of tubulin. We test our approach on the 3D images of HeLa cells previously used to develop the model, and then use it to compare microtubule distributions in different cell lines. Figure 2 provides an overview of the framework introduced in this paper. There are two sub-systems. One is for generating synthetic images of microtubules, and the other is for estimating the microtubule model parameters for real images through comparison with the synthetic images. We first obtained 2D fluorescence microscopy images for eleven cell lines. Each image contains two channels, one for microtubule staining and the other for nuclear staining. The images are segmented to find individual cell and nuclear boundaries. For each cell, we estimate a Point Spread Function (PSF), centrosome location and single microtu-bule intensity. On the basis of the segmented 2D cell and nuclear shapes, approximate 3D cell and nuclear morphologies are generated. Given the model 23727046 (Figure 1) and ranges of allowed values of its parameters (number of microtubules (N), mean of the length distribution (mu) and collinearity (a)), synthetic images of microtubule distributions are generated for each 3D morphology for each combination of allowed parameter values. Each raw synthetic image is then convolved with the estimated PSF and multiplied with the estimated single microtubule intensity to make it comparable to the real image. Numerical features are then calculated on every real cell image and the synthetic images for it. The matching method then selects one set of parameters for which the synthetic image is the closest to the real image in the feature space. Using this indirect method, we estimate the model parameters for 2D images from eleven human cell lines, and analyze the resulting parameters.Results 3D Cell and Nuclear Shape Generation from a 2D Slice of Microtubule Channel and Nucleus ChannelIn our earlier work, we described an indirect approach to estimate parameters of a generative model of microtubules that was conditioned on the shape of the cell and the nucleus [8]. These shapes were estimated from a 3D confoca.