Zed strains with mutations in ATPsynthase genes, which is composed of

Zed strains with mutations in ATPsynthase genes, which is composed of a soluble F1 component that catalyzes ATP-synthesis or hydrolysis, and of a transmembrane F0 component that mediates proton translocation across the inner membrane: Datp12 cells that lack a factor required for F1 assembly, display reduced ATP-synthase and accumulate inclusion bodies containing unassembled F1-proteins [27] and three strains with deleted or mutated ATP6 (Datp6, atp6-L183R, atp6-L247R) that we have characterized in the past (Table 2). The atp6-L183R mutation [3] is homologous to human T8993G/L156R, the most frequent mutation associated to neurogenic ataxia retinitis pigmentosa (NARP). The atp6-L247R mutation [4] is homologous to human SIS-3 T9176G/L217R, which is associated to maternallyinherited Leigh syndrome (MILS), the most severe form of NARP. It is important to note that the mutation of genes encoding components or assembly factors of ATP-synthase also display lower levels of complex IV (Table 2) and sometimes also of complex III [2,4,27,28]. We characterized the bioenergetic properties of these strains under the culture conditions of the fusion assay, which relies on successive growth on media containing fermentable substrates (Gal and Glc) that allow the metabolic compensation by glycolysis required for growth and conjugation of OXPHOS-deficient strains. Fig. 2A shows the respiration rates of cells in the DprE1-IN-2 web presence of ethanol alone (all strains), after inhibition of ATP-synthase with triethyl-tin (tet: WT, atp6-L183R, atp6-L247R) or after addition of the protonophore CCCP (all strains). Wild-type cells revealed TET-sensitive and CCCP-stimulated respiration, as expected. Strains carrying point-mutations in the ATP-synthase gene (atp6L183R, atp6-L247R) retained a low respiratory capacity that was insensitive to TET (due to defective ATP-synthase) but could be stimulated with CCCP. The other mutants depicted residual, CCCP-insensitive O2 consumption (Fig. 2A). We next analyzed the cellular levels of ATP and ADP as well as the ATP/ADP ratios (Fig 2B). The similar values obtained in wild-type and mutant cells confirmed efficient metabolic compensation by glycolysis. Of note, the ATP/ADP ratios (Fig. 2B: 4,2?,8) are similar to 1081537 those found in wild-type cells grown under fermentative conditions (,4) and lower than those attained by wild-type cells relying on OXPHOS (11?7; [23]).Next, we setup to analyze the mitochondrial inner membrane potential DYm and the content of reactive oxygen species. Cells were incubated with rhodamine 123 (rh123), a fluorescent probe that accumulates in mitochondria in a DYm-dependent manner and dihydroethidium (DHE), a blue-fluorescent probe that is oxidized to green-fluorescent ethidium by superoxide. The amount of accumulated rh123 and ethidium, which are proportional to the DYm, and the superoxide content, respectively, was analyzed by flow cytometry. The mean and the median fluorescence intensity (Fig. 2C, D) and the fluorescence distributions (Supp. Fig. S2) revealed lower amounts of rh123 and ethidium in OXPHOS-deficient strains, pointing to lower DYm and ROS-contents.Mitochondrial Fusion is Inhibited in Cells with Genetic OXPHOS DefectsWe first studied fusion in yeast strains that were devoid of mtDNA (r0) or of the mitochondrial gene COX2 (Dcox2). Visualization of mitochondrially targeted GFP (mtGFP) revealed filamentous mitochondrial morphology in r0 and in Dcox2 cells (Supp. Fig. S3). However, fusion assays with matrix-targ.Zed strains with mutations in ATPsynthase genes, which is composed of a soluble F1 component that catalyzes ATP-synthesis or hydrolysis, and of a transmembrane F0 component that mediates proton translocation across the inner membrane: Datp12 cells that lack a factor required for F1 assembly, display reduced ATP-synthase and accumulate inclusion bodies containing unassembled F1-proteins [27] and three strains with deleted or mutated ATP6 (Datp6, atp6-L183R, atp6-L247R) that we have characterized in the past (Table 2). The atp6-L183R mutation [3] is homologous to human T8993G/L156R, the most frequent mutation associated to neurogenic ataxia retinitis pigmentosa (NARP). The atp6-L247R mutation [4] is homologous to human T9176G/L217R, which is associated to maternallyinherited Leigh syndrome (MILS), the most severe form of NARP. It is important to note that the mutation of genes encoding components or assembly factors of ATP-synthase also display lower levels of complex IV (Table 2) and sometimes also of complex III [2,4,27,28]. We characterized the bioenergetic properties of these strains under the culture conditions of the fusion assay, which relies on successive growth on media containing fermentable substrates (Gal and Glc) that allow the metabolic compensation by glycolysis required for growth and conjugation of OXPHOS-deficient strains. Fig. 2A shows the respiration rates of cells in the presence of ethanol alone (all strains), after inhibition of ATP-synthase with triethyl-tin (tet: WT, atp6-L183R, atp6-L247R) or after addition of the protonophore CCCP (all strains). Wild-type cells revealed TET-sensitive and CCCP-stimulated respiration, as expected. Strains carrying point-mutations in the ATP-synthase gene (atp6L183R, atp6-L247R) retained a low respiratory capacity that was insensitive to TET (due to defective ATP-synthase) but could be stimulated with CCCP. The other mutants depicted residual, CCCP-insensitive O2 consumption (Fig. 2A). We next analyzed the cellular levels of ATP and ADP as well as the ATP/ADP ratios (Fig 2B). The similar values obtained in wild-type and mutant cells confirmed efficient metabolic compensation by glycolysis. Of note, the ATP/ADP ratios (Fig. 2B: 4,2?,8) are similar to 1081537 those found in wild-type cells grown under fermentative conditions (,4) and lower than those attained by wild-type cells relying on OXPHOS (11?7; [23]).Next, we setup to analyze the mitochondrial inner membrane potential DYm and the content of reactive oxygen species. Cells were incubated with rhodamine 123 (rh123), a fluorescent probe that accumulates in mitochondria in a DYm-dependent manner and dihydroethidium (DHE), a blue-fluorescent probe that is oxidized to green-fluorescent ethidium by superoxide. The amount of accumulated rh123 and ethidium, which are proportional to the DYm, and the superoxide content, respectively, was analyzed by flow cytometry. The mean and the median fluorescence intensity (Fig. 2C, D) and the fluorescence distributions (Supp. Fig. S2) revealed lower amounts of rh123 and ethidium in OXPHOS-deficient strains, pointing to lower DYm and ROS-contents.Mitochondrial Fusion is Inhibited in Cells with Genetic OXPHOS DefectsWe first studied fusion in yeast strains that were devoid of mtDNA (r0) or of the mitochondrial gene COX2 (Dcox2). Visualization of mitochondrially targeted GFP (mtGFP) revealed filamentous mitochondrial morphology in r0 and in Dcox2 cells (Supp. Fig. S3). However, fusion assays with matrix-targ.