An mammalian collagen [8,9]. Marine collagen, for example that from sponges, jellyfish, squids, octopuses, cuttlefish, and fish skin, bone, and scales, comes from both marine vertebrates and invertebrates [9,10]. There is fantastic demand for marine collagen, andPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI, Basel, GS-626510 web Switzerland. This article is definitely an open access short article distributed beneath the terms and situations of your Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ four.0/).Mar. Drugs 2021, 19, 597. https://doi.org/10.3390/mdhttps://www.mdpi.com/journal/marinedrugsMar. Drugs 2021, 19,two ofthis is now the primary supply of collagen globally. Sourour Addad et al. (2011) obtained collagen from Jellyfish , Tziveleka et al. (2017) isolated the collagen from marine sponges Axinella cannabina and Thromboxane B2 Epigenetics Suberites carnosus  and skin and Cruz-L ez et al. (2018) extracted collagen from gulf corvina skin and swim bladder . As reported within the literature, the marine collagen market place is anticipated to attain USD 983.84 million by 2025, using a compound annual growth price of 7.4 . Marine collagen, compared with collagen from terrestrial sources, is more quickly extracted , has higher biocompatibility , is with out the dangers of animal ailments and pathogens; has a higher absorption capacity (as much as 1.five occasions a lot more effective entry into the body), and will not be related with religious and ethical restrictions [14,15]. This delivers an opportunity for fish scales. Namely, fish scales will be the waste product in the fish processing sector, and they represent on typical 2 of fish physique weight . The poor biodegradability of scales makes them tough to be managed as waste . However, scales are a safe and great source of marine variety I collagen . Therefore, the extraction of type I collagen from scales might be helpful with regards to each economic and environmental advantages, and it could possibly drive the improvement of new industries. Type I collagen from scales has gained growing interest, and scales are extensively regarded as a promising source of collagen . Many thriving extractions of collagen from scales have already been reported, like tilapia scales collagen , gourami scales collagen , and miiuy croakers scales collagen . Lizardfish (Synodus macrops) is a prevalent financial fish species in China, and there have already been no studies about lizardfish scales collagen. Thus, within this study, we isolated collagen from lizardfish scales by using acid and enzymatic extraction solutions; characterized the physicochemical properties, structural properties, and thermal stability of acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC), and investigated the rheological properties, and cell viability, all of which could possibly present helpful information and facts for the development and application of marine collagen. 2. Results two.1. Collagen Yield The collagen from lizardfish scales was prepared using acid extraction and enzymatic extraction separately. The yield of ASC and PSC was four.2 0.2 (based on a dry weight basis) and 4.7 0.1 (dry weight), respectively. two.two. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) The electrophoretic patterns of ASC and PSC from lizardfish scales are illustrated in Figure 1. It’s clear that ASC and PSC show comparable electrophoretic patterns as each consis.