T in the LP and increased gradually toward the SC layer. The distinction in expression

T in the LP and increased gradually toward the SC layer. The distinction in expression among the diverse layers nonetheless didn’t attain statistical significance (Fig. 5A). Wnt 5b–Wnt 5b level of expression was also substantially different between the layers (P0.005; Fig. 5B). It was highest in the LP and was much more than 15-folds and SSTR4 Activator web 6-folds TLR4 Agonist web greater than the IC layer (P0.005) and SC layers (P0.02), respectively. Expression within the BC layer was not different from the LP but was much more than 8-folds (P0.008) and 3-folds (P0.03) greater than the intermediate and SC layers, respectively. Wnt 5a–Wnt 5a expression was also determined to be statistically distinctive in between the diverse layers (P0.03; Fig. 6A) but its expression pattern was distinctive from Wnt 5b. Expression within the IC layer was highest and more than 4-folds greater than the BC layer (P0.002). Pair-wise comparison amongst the other layers didn’t attain statistical significance. Wnt Receptor Expression FZD 1–Comparison with the expression of Wnt receptor, FZD 1 revealed important variations among the diverse layers (P0.04, Fig. 7A). FZD 1 was expressed greatest in the LP and lowest within the SC layer. Expression within the LP was additional than 8-folds higher than the SC layer (P0.02). Comparison involving the other layers didn’t reach statistical significance. FZD 6–The expression of FZD 6 was greater in the LP and BCs compared with all the IC layer and SC layer. The distinction in expression between these layers didn’t attain statistical significance (Fig. 7B). LRP 6–The expression level of LRP 6 was substantially distinctive amongst the distinct layers (P0.02, Fig. 7C). Expression inside the LP was highest followed by the BC layer and reduce levels of expression were observed inside the IC and SC layers. The expression within the LP was extra than 11-folds higher than the IC layer (P0.01). Pair-wise comparison amongst the other layers did not attain statistical significance. Wnt Modulating Protein Expression Dkk1–The expression of Dkk1 was predominantly inside the SC layer and decreased abruptly inside the other layers (Figs. 8A, B). ANOVA showed significant differences within the expressionAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptJ Clin Gastroenterol. Author manuscript; out there in PMC 2016 March 29.Ali et al.Pageof Dkk1 among cell layers (P0.002). Pair-wise comparison demonstrated that the expression of Dkk1 in SC layer was much more than 100-fold greater than all of the other layers (P0.001). Comparison amongst other cell layers didn’t reach statistical significance. Dkk3–In contrast to Dkk1, the expression of Dkk3 decreased precipitously from the LP towards the SC layer (Fig. 9A). ANOVA showed important variations in expression of Dkk3 among cell layers (P0.001). Pair-wise comparison demonstrated that the expression of Dkk3 in the LP was extra than 25-folds greater than the IC layer and 7-folds greater than SC layer (P0.003). Dkk3 expression within the BC layer was a lot more than 3-folds higher than that determined in the SC layer and the IC layers (P0.005). Expression in the LP was also significantly higher than the BC layer (P0.03). Dkk4–The expression of Dkk4 alternatively enhanced precipitously and substantially in the LP for the SC layer (Fig. 10A). ANOVA showed significant differences in the expression of Dkk4 among the cell layers (P0.001). Pair-wise comparison demonstrated that Dkk4 expression within the SC layer was additional than 30-folds higher than the LP and also the BC layer (P0.001) and.