Enes, except blaADC-like, as well as 1 kb upstream of your gene when

Enes, except blaADC-like, and also 1 kb upstream with the gene when an insertion element was detected by utilizing ISAba1-specific primers or by applying inverse PCR. All isolates scored constructive for the intrinsic blaOXA-51-like and blaADC-like genes. Eleven isolates (38 ) scored optimistic for blaOXA-23-like and four isolates (14 ) for blaOXA-40-like, which upon sequencing appeared to encode OXA-23 and OXA-40, respectively. These isolates had been all resistant to each tested carbapenems, whereas all other isolates have been susceptible or showed intermediate levels of resistance (Table 1). A blaCMY-2-like gene (encoding plasmid-mediated AmpC-type cephalosporinase) was detected in 1 isolate and encoded CMY-30, a close homologue of CMY-2 with comparable activity (13). To our expertise, the presence of blaCMY-2-like inside a. baumannii has not been reported previously. A blaPER-1-like gene was detected in isolates ten, 20, and 22, which upon sequencing appeared to encode PER-1 using the insertion element ISPa12 upstream on the gene (18). blaGES-1-like was detected in isolate 29 and encoded the GES-11 variant, which hasReceived 7 March 2015 Accepted 14 March 2015 Accepted manuscript posted on the internet 18 March 2015 Citation Trip H, Mende K, Majchrzykiewicz-Koehorst JA, Sedee NJA, Hulst AG, Jansen H-J, Murray CK, Paauw A. 2015. Simultaneous identification of a number of -lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry. J Clin Microbiol 53:1927930. doi:ten.1128/JCM.00620-15. Editor: E. Munson Address correspondence to Hein Trip, [email protected]. * Present address: Joanna A. Majchrzykiewicz-Koehorst, Sanquin, Amsterdam, The Netherlands. Supplemental material for this article could be identified at http://dx.doi.org/10.1128 /JCM.00620-15. Copyright 2015, American Society for Microbiology. All Rights Reserved. doi:ten.1128/JCM.00620-June 2015 Volume 53 NumberJournal of Clinical Microbiologyjcm.asm.orgTrip et al.jcm.asm.orgTABLE 1 MICs of imipenem, meropenem, and ceftazidime for 29 clinical A. baumannii isolates, identification of -lactamase genes and upstream IS components by PCR, and LC-MS/MS detection of -lactamase proteinsblaOXA-23-like/OXA-23-like PCR ISAba1 ISAba1 ISAba1 ISAba1 blaCMY-2-like ISAba1 ISAba1 ISAba1 ISAba1 blaPER-1-like ISPa12b PER-1-like CMY-2-like IS LC-MS/MS PCR IS LC-MS/MS PCR IS LC-MS/MS PCR IS blaOXA-40-like/OXA-40-like blaADC-like/ADC-like Other -lactamases LC-MS/MSMIC (mg/liter)a LC-MS/MSblaOXA-51-like/OXA-51-likeIsolate no.Rhein Autophagy IPMMEMCAZPCRISPFGE typeISAbaJournal of Clinical MicrobiologyISAba16 ISAbablaPER-1-like blaPER-1-likeISPa12 ISPaPER-1-like PER-1-likeISAbaISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 two three four 5 6 7 eight 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 ISAba1 blaGES-1-like4 4 two 4 eight four 4 4 4 4 4 two four 32 32 32 32 32 32 32 32 32 32 32 32 32 four 328 4 1 eight four 4 four 2 four 1 4 0,5 four 16 16 16 16 16 16 16 16 16 16 16 16 16 four 1664 64 64 64 64 64 64 64 64 64 8 eight 8 64 64 64 64 16 32 64 32 64 32 64 64 64 64 32ISAbaGES-1-like10 ten 3 7 6 7 13 12 10 eight 14 2 9 1 1 1 1 12 12 15 12 15 12 5 five five 7 128 mg/liter; intermediate, 16 mg/June 2015 Volume 53 NumberCLSI breakpoints for imipenem and meropenem were as follows: susceptible, 4 mg/liter; intermediate, eight mg/liter; resistant, 16 mg/liter.Adenosine 3′,5′-diphosphate disodium Biological Activity Breakpoints for ceftazidime have been as follows: susce.PMID:24732841