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Ryanodine receptor monoclonal antibody (C3-33)
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The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the “foot” region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).254750-02-2 Biological Activity Figure: Immunolocalization of ryanodine receptor 1 (RyR1) in rat brain hippocampus using Monoclonal Antibody to Ryanodine Receptor (C3-33) .1970972-74-7 site Figure: Immunolocalization of ryanodine receptor 1 (RyR1) in rat brain hippocampus using Monoclonal Antibody to Ryanodine Receptor (C3-33) .PMID:30855902
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| Alternative Name RyR | Application Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB | Application Notes Detects a band of 565kDa by Western blot. In non-mammalian vertebrates, a doublet is seen at 565kDa representing the α and β isoforms of the receptor. | Clone C3-33 | Crossreactivity Cross-reacts weakly with RyR-1. | Formulation Liquid. In PBS containing 0.05% sodium azide. | Host Mouse | Immunogen Dog cardiac RyR (ryanodine receptor). | Isotype IgG1 | Recommendation Dilutions/Conditions Immunocytochemistry (1:100-1:200)Immunohistochemistry (frozen sections, 1µg/ml)Immunohistochemistry (paraffin sections, 1µg/ml)Western Blot (1µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Amphibian, Chicken, Dog, Fish, Guinea pig, Human, Mouse, Rabbit, Rat | UniProt ID Q92736 (human RYR2) | Unit of Measure (UM) µg
