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Major MEFs (only two passages in tradition) downregulated for Mybbp1a showed a speedy entry into senescence in contrast with cells treated with non-focusing on vector (NT) in a extended phrase proliferation protocol (Figure 2A,B)

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Colony detection was performed by staining cells with three-(4,five-dimethylthiazol-2-ye)-2,five-diphenyltetrazolium bromide (MTT, 1:10 in PBS...