This phenotype is equivalent to that observed for other ARKO designs [thirty,31,32]. MMTV-Cre induced recombination of the Arfl allele resulting in deletion of exon 2 was verified by PCR investigation of MARKO genomic DNA isolated from partially dissociated mammary glands (Figure 1B)
DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA) and the remaining cells were being...